| Product Name |
Recombinant Human Ribonuclease H (RNASEH/RNASEH1) Protein |
| Product Overview |
This recombinant human Ribonuclease H (RNASEH/RNASEH1) protein includes amino acids 1-286aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
O60930 |
| Recommended Name |
Ribonuclease H1 |
| Gene Name |
RNASEH1 |
| Synonyms |
Rnase-H; RNASEH1; RNH1; Ribonuclease H type II |
| Species |
Human |
| Predicted Molecular Mass |
62 kDa |
| Expression System |
E.coli |
| Expression Range |
1-286aa |
| Tag |
N-His-GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
RNase H family |
| Associated Diseases |
Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2) |
| Tissue Specificity |
Ubiquitous. |
