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AstorScientific | SKU: AirTaq-M1-50

Air-Taq™ Polymerase Complete qPCR Mix (2x)

Name: Air-Taq™ Polymerase Complete qPCR Mix (2x)
Brand: AstorScientific
SKU: AirTaq-M1-50
Price: 35.0 USD
Availability: InStock

$35.00

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Product Description

Performance and Benchmarking

Airobes' qPCR mix shows exceptional repeatability. (left) The coefficient of variation was <1% for starting DNA amounts ranging from 2ng – 20 femtograms. n = 6 replicates. (right) The coefficient of variation was was <1% for DNA product sizes of 150bp, 500bp, 1000bp, and 2000bp. n = 6 replicates.

Includes Hot-Start Taq Polymerase

High-performance 2x concentrated qPCR master mix with SYBR® Green I and low ROX. Ideal for quantitative PCR applications including cDNA amplification, genotyping, and more.

About This Product

Airobes recombinant Taq DNA Polymerase is a thermostable enzyme with carbon molecules sourced from CO₂ in the air. Taq Polymerase synthesizes DNA from single-stranded templates in the presence of dNTPs, a magnesium cofactor, and primers. This recombinant enzyme is cloned from Thermus aquaticus DNA polymerase and expressed in a proprietary strain of E. coli. It consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and 5' to 3' exonuclease activity.

Key Features

Convenience: Ready-to-use 2x PCR Master Mix. Simply add the provided DNA, primers, and water

Sustainable: Protein carbon sourced from captured CO₂

Balanced Yield and Specificity: Optimized buffer system with potassium chloride and ammonium sulfate

High Efficiency: >90%

Highly Repeatable: Coefficient of Variation <1% for copy numbers ranging from 10³ - 10⁸ and amplicon size ranging from 100 - 2000 base pairs.

Linear: R² > 0.99 across a 6-log dynamic range spanning 10³ - 10⁸ copies

Applications

qPCR (quantitative Polymerase Chain Reaction)

Endpoint PCR

T/A Cloning

Genotyping

Environmental and Microbial Screening by 16S rRNA

cDNA Amplification

Educational and Teaching Labs

Technical Notes

One unit of Taq Polymerase catalyzes the incorporation of 10 nmol of dNTPs (deoxyribonucleotides) into a polynucleotide fraction in 30 min at 74°C
Includes hot-start Taq DNA Polymerase. Use a 15 minute activation time at 94-95°C
Recommended starting copy number between 10³ - 10⁸ copies.
For best performance, we recommend an amplicon size ≤300bp. However, DNA product sizes up to 2kb have been verified to amplify with this mix.

For 2kb amplicons, use a 2-3 minute extension time and (at least) 35 cycles.
Contains SYBR® Green I and (low) ROX Passive Reference Dye for qPCR applications
Includes nuclease-free water for convenient reaction setup
We have performed degradation testing and guarantee product functionality when stored at -20°C upon delivery.

Specifications (1x)

Tris-HCl:20 mM Tris-HCl (pH 8.0 at 25°C)

potassium Chloride:40 mM KCl

dNTPs:200 μM each

magnesium Chloride:3.2 mM MgCl₂

Hot-Start Taq Polymerase:1.2 Units / 20 μL

PCR Enhancers

Tween 20

Betaine

DMSO

Glycerol

Sucrose

Polyethylene glycol

BSA

Dyes

Thiazole Green (SYBR® Green I)

(Low) ROX Passive Reference Dye

Air-Taq™ Polymerase Complete qPCR Mix (2x)

Care information

Delivery and Shipping

Payment & Security

Product Description

Performance and Benchmarking

About This Product

Key Features

Applications

Technical Notes

Specifications (1x)

PCR Enhancers

Dyes

Product Protocol

SDS

Tips and Tricks

COA

Master Mix Components

Product Reviews

Customer Reviews

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