| Product Name |
Recombinant Mouse Growth Differentiation Factor 15 (GDF15) Protein |
| Product Overview |
This recombinant mouse Growth Differentiation Factor 15 (GDF15) protein includes amino acids 189-303aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9Z0J7 |
| Recommended Name |
Growth/differentiation factor 15 |
| Gene Name |
Gdf15 |
| Synonyms |
Growth/differentiation factor 15 precursor, MIC-1, NAG-1, SBF |
| Species |
Mouse |
| Predicted Molecular Mass |
16 kDa |
| Expression System |
E.coli |
| Expression Range |
189-303aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling. |
| Subcellular Location |
Secreted. |
| Protein Family |
TGF-beta family |
| Tissue Specificity |
Highly expressed in liver. Detected in plasma (at protein level). Expressed by cardiomyocytes, expression is highly increased in heart diseases. Also detected in subcutaneous fat. |
