| Product Name |
Recombinant Human Vascular Cell Adhesion Molecule 1 (VCAM1) Protein |
| Product Overview |
This recombinant human Vascular Cell Adhesion Molecule 1 (VCAM1) protein includes amino acids 109-318aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P19320 |
| Recommended Name |
Vascular cell adhesion protein 1 |
| Gene Name |
VCAM1 |
| Synonyms |
Vascular cell adhesion molecule 1, CD106; DKFZp 779G2333; INCAM-100; MGC99561 |
| Species |
Human |
| Predicted Molecular Mass |
53 kDa |
| Expression System |
E.coli |
| Expression Range |
109-318aa |
| Tag |
N-6His+GST |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Adhesion |
| Target Function |
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. |
| Subcellular Location |
Membrane; Single-pass type I membrane protein. |
| Tissue Specificity |
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. |
