| Product Name |
Recombinant Human Ubiquitin Conjugating Enzyme E2I (UBE2I) Protein |
| Product Overview |
This recombinant human Ubiquitin Conjugating Enzyme E2I (UBE2I) protein includes amino acids 33-158aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P63279 |
| Recommended Name |
SUMO-conjugating enzyme UBC9 |
| Gene Name |
UBE2I |
| Synonyms |
UBE2I, UBC9, UBCE9, Ubc9, Human ubiquitin conjugating enzyme 9, EC 6.3.2, SUMO-protein ligase, Ubiqu |
| Species |
Human |
| Predicted Molecular Mass |
18 kDa |
| Expression System |
E.coli |
| Expression Range |
33-158aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Cycle |
| Target Function |
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. |
| Subcellular Location |
Nucleus. Cytoplasm. |
| Protein Family |
Ubiquitin-conjugating enzyme family |
| Tissue Specificity |
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
