| Product Name |
Recombinant Human Tubulin Beta 3 (TUBb3) Protein |
| Product Overview |
This recombinant human Tubulin Beta 3 (TUBb3) protein includes amino acids 222-438aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q13509 |
| Recommended Name |
Tubulin beta-3 chain |
| Gene Name |
TUBB3 |
| Synonyms |
Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TUBB4 |
| Species |
Human |
| Predicted Molecular Mass |
54 kDa |
| Expression System |
E.coli |
| Expression Range |
222-438aa |
| Tag |
N-6His+GST |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Subcellular Location |
Cytoplasm, cytoskeleton. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium. |
| Protein Family |
Tubulin family |
| Associated Diseases |
Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A); Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1) |
| Tissue Specificity |
Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
