| Product Name |
Recombinant Human SAE1 Protein |
| Product Overview |
This recombinant human SAE1 protein includes amino acids 1-346aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9UBE0 |
| Recommended Name |
SUMO-activating enzyme subunit 1 |
| Gene Name |
SAE1 |
| Synonyms |
SUMO1 activating enzyme subunit 1, AOS1, HSPC140, SUA1, UBLE1A |
| Species |
Human |
| Predicted Molecular Mass |
42.2 kDa |
| Expression System |
E.coli |
| Expression Range |
1-346aa |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Subcellular Location |
Nucleus. |
| Protein Family |
Ubiquitin-activating E1 family |
| Tissue Specificity |
Expression level increases during S phase and drops in G2 phase (at protein level). |
