| Product Name |
Recombinant Human Purine Nucleoside Phosphorylase (PNP) Protein |
| Product Overview |
This recombinant human Purine Nucleoside Phosphorylase (PNP) protein includes amino acids 1-289aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P00491 |
| Recommended Name |
Purine nucleoside phosphorylase |
| Gene Name |
PNP |
| Synonyms |
Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP. |
| Species |
Human |
| Predicted Molecular Mass |
36 kDa |
| Expression System |
E.coli |
| Expression Range |
1-289aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
PNP/MTAP phosphorylase family |
| Associated Diseases |
Purine nucleoside phosphorylase deficiency (PNPD) |
| Tissue Specificity |
Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology. |
