| Product Name |
Recombinant Human Endoplasmic Reticulum Resident 57 (ERp57/PDIA3) Protein |
| Product Overview |
This recombinant human Endoplasmic Reticulum Resident 57 (ERp57/PDIA3) protein includes amino acids 162-416aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P30101 |
| Recommended Name |
Protein disulfide-isomerase A3 |
| Gene Name |
PDIA3 |
| Synonyms |
ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC |
| Species |
Human |
| Predicted Molecular Mass |
33 kDa |
| Expression System |
E.coli |
| Expression Range |
162-416aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Subcellular Location |
Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. |
| Protein Family |
Protein disulfide isomerase family |
| Tissue Specificity |
Detected in the flagellum and head region of spermatozoa (at protein level). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level). |
