| Product Name |
Recombinant Human Mitogen Activated Kinase Kinase 3 (MAP2K3) Protein |
| Product Overview |
This recombinant human Mitogen Activated Kinase Kinase 3 (MAP2K3) protein includes amino acids 99-343aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P46734 |
| Recommended Name |
Dual specificity mitogen-activated protein kinase kinase 3 |
| Gene Name |
MAP2K3 |
| Synonyms |
Dual specificity mitogen-activated protein kinase kinase 3, MAPKK3, MEK3, MKK3, PRKMK3 |
| Species |
Human |
| Predicted Molecular Mass |
32 kDa |
| Expression System |
E.coli |
| Expression Range |
99-343aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. |
| Protein Family |
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily |
| Associated Diseases |
Defects in MAP2K3 may be involved in colon cancer. |
| Tissue Specificity |
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
