| Product Name |
Recombinant Human KRT8 Protein |
| Product Overview |
This recombinant human KRT8 protein includes amino acids 1-483aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P05787 |
| Recommended Name |
Keratin, type II cytoskeletal 8 |
| Gene Name |
KRT8 |
| Synonyms |
Keratin, type II cytoskeletal 8, keratin 8, CARD2, CK-8, CK8, CYK8, K2C8, K8, KO |
| Species |
Human |
| Predicted Molecular Mass |
56 kDa |
| Expression System |
E.coli |
| Expression Range |
1-483aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Subcellular Location |
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. |
| Protein Family |
Intermediate filament family |
| Associated Diseases |
Cirrhosis (CIRRH) |
| Tissue Specificity |
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
