| Product Name |
Recombinant Human ISCU Protein |
| Product Overview |
This recombinant human ISCU protein includes amino acids 35-167aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9H1K1 |
| Recommended Name |
Iron-sulfur cluster assembly enzyme ISCU |
| Gene Name |
ISCU |
| Synonyms |
Iron-sulfur cluster assembly enzyme ISCU, HML, hnifU, ISU2, NIFU, NIFUN |
| Species |
Human |
| Predicted Molecular Mass |
16.7 kDa |
| Expression System |
E.coli |
| Expression Range |
35-167aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism, Signal Transduction |
| Target Function |
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm. |
| Subcellular Location |
[Isoform 1]: Mitochondrion.; [Isoform 2]: Cytoplasm. Nucleus. |
| Protein Family |
NifU family |
| Associated Diseases |
Myopathy with exercise intolerance Swedish type (MEIS) |
| Tissue Specificity |
Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta. |
