Product Name |
Recombinant Human Histone H3 (H3F3A) Protein |
Product Overview |
This Recombinant Human Histone H3 (H3F3A) Protein with amino acids Met1-Ala136 is expressed in E.coli. The protein is supplied lyophilized and formulated in Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose.It has a purity >95% as determined by SDS-PAGE. |
Target Uniprot Id |
B2R4P9 |
Synonyms |
Histone H3.3, H3.3A, H3F3 |
Species |
Human |
Predicted Molecular Mass |
17.7 kDa |
Expression System |
E.coli |
Expression Range |
1-136aa |
Tag |
N-6His |
Purity |
>95% |
Formulation |
Lyophilized |
Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Applications |
Positive Control; Immunogen; SDS-PAGE; WB |