| Product Name |
Recombinant Human Histone Deacetylase 8 (HDAC8) Protein |
| Product Overview |
This recombinant human Histone Deacetylase 8 (HDAC8) protein includes amino acids 1-377aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9BY41 |
| Recommended Name |
Histone deacetylase 8 |
| Gene Name |
HDAC8 |
| Synonyms |
Histone deacetylase 8, CDA07, HD8, HDACL1, RPD3. |
| Species |
Human |
| Predicted Molecular Mass |
46 kDa |
| Expression System |
E.coli |
| Expression Range |
1-377aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Transcription |
| Target Function |
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. |
| Subcellular Location |
Nucleus. Cytoplasm. Note=Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. |
| Protein Family |
Histone deacetylase family, HD type 1 subfamily |
| Associated Diseases |
Cornelia de Lange syndrome 5 (CDLS5); Wilson-Turner X-linked mental retardation syndrome (WTS) |
| Tissue Specificity |
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. |
