Product Name |
Recombinant Human Granzyme B (GZMB) Protein |
Product Overview |
This recombinant human Granzyme B (GZMB) protein includes amino acids 21-247aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
Target Uniprot Id |
P10144 |
Recommended Name |
Granzyme B |
Gene Name |
GZMB |
Synonyms |
Granzyme B, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT |
Species |
Human |
Predicted Molecular Mass |
31 kDa |
Expression System |
E.coli |
Expression Range |
21-247aa |
Tag |
N-6His |
Purity |
>90% |
Formulation |
Lyophilized |
Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
Research Area |
Immunology |
Target Function |
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. |
Subcellular Location |
Secreted. Cytolytic granule. |
Protein Family |
Peptidase S1 family, Granzyme subfamily |