| Product Name |
Recombinant Human GABA-A Receptor Associated Like 2 (GABARAPL2) Protein |
| Product Overview |
This recombinant human GABA-A Receptor Associated Like 2 (GABARAPL2) protein includes amino acids 1-117aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P60520 |
| Recommended Name |
Gamma-aminobutyric acid receptor-associated protein-like 2 |
| Gene Name |
GABARAPL2 |
| Synonyms |
Gamma-aminobutyric acid receptor-associated protein-like 2, ATG8, ATG8C, GATE-16, GATE16, GEF2 |
| Species |
Human |
| Predicted Molecular Mass |
17 kDa |
| Expression System |
E.coli |
| Expression Range |
1-117aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Transport |
| Target Function |
Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. |
| Subcellular Location |
Cytoplasmic vesicle, autophagosome. Endoplasmic reticulum membrane. Golgi apparatus. |
| Protein Family |
ATG8 family |
| Tissue Specificity |
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. |
