| Product Name |
Recombinant Human EPHX1 Protein |
| Product Overview |
This recombinant human EPHX1 protein includes amino acids 21-455aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P07099 |
| Recommended Name |
Epoxide hydrolase 1 |
| Gene Name |
EPHX1 |
| Synonyms |
Epoxide hydrolase 1, microsomal (xenobiotic), Epoxide hydrolase 1, microsomal (xenobiotic), MEH, EPH |
| Species |
Human |
| Predicted Molecular Mass |
52.2 kDa |
| Expression System |
E.coli |
| Expression Range |
21-455aa |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism, Cell Biology |
| Target Function |
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol. |
| Subcellular Location |
Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. |
| Protein Family |
Peptidase S33 family |
| Associated Diseases |
Familial hypercholanemia (FHCA) |
| Tissue Specificity |
Found in liver. |
