| Product Name |
Recombinant Human Epidermal Growth Factor (EGF) Protein |
| Product Overview |
This recombinant human Epidermal Growth Factor (EGF) protein includes amino acids 971-1023aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P01133 |
| Recommended Name |
Pro-epidermal growth factor |
| Gene Name |
EGF |
| Synonyms |
Epidermal growth factor, Urogastrone |
| Species |
Human |
| Predicted Molecular Mass |
10 kDa |
| Expression System |
E.coli |
| Expression Range |
971-1023aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction, Cancer |
| Target Function |
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Subcellular Location |
Membrane; Single-pass type I membrane protein. |
| Associated Diseases |
Hypomagnesemia 4 (HOMG4) |
| Tissue Specificity |
Expressed in kidney, salivary gland, cerebrum and prostate. |
