| Product Name |
Recombinant Human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 (DMP1) Protein |
| Product Overview |
This recombinant human Dentin Matrix Acidic Phosphoprotein 1/DMP-1 (DMP1) protein includes amino acids 17-513aa of the target gene is expressed in HEK293 Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q13316 |
| Recommended Name |
Dentin matrix acidic phosphoprotein 1 |
| Gene Name |
DMP1 |
| Synonyms |
Dentin Matrix Acidic Phosphoprotein 1; DMP-1; Dentin Matrix Protein 1; DMP1 |
| Species |
Human |
| Predicted Molecular Mass |
55.0 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
17-513aa |
| Tag |
C-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. |
| Subcellular Location |
Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. |
| Associated Diseases |
Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1) |
| Tissue Specificity |
Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. |
