| Product Name |
Recombinant Human Collapsin Response Mediator 1 (CRMP1) Protein |
| Product Overview |
This recombinant human Collapsin Response Mediator 1 (CRMP1) protein includes amino acids 1-250aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q14194 |
| Recommended Name |
Dihydropyrimidinase-related protein 1 |
| Gene Name |
CRMP1 |
| Synonyms |
Dihydropyrimidinase-related protein 1 isoform2, CRMP-1, DPYSL1, DRP-1, DRP1, uLIP-3 |
| Species |
Human |
| Predicted Molecular Mass |
28 kDa |
| Expression System |
E.coli |
| Expression Range |
1-250aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Neuroscience |
| Target Function |
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone. Involved in invasive growth and cell migration. May participate in cytokinesis. |
| Subcellular Location |
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cell projection, growth cone. Cytoplasm, cytoskeleton. Perikaryon. |
| Protein Family |
Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family |
| Tissue Specificity |
Brain. |
