| Product Name |
Recombinant Human Teratocarcinoma-Derived Growth Factor 1/TDGF1/Cripto Protein |
| Product Overview |
This recombinant human Teratocarcinoma-Derived Growth Factor 1/TDGF1/Cripto protein includes amino acids 31-169aa of the target gene is expressed in HEK293 Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P13385 |
| Recommended Name |
Protein Cripto |
| Gene Name |
CRIPTO |
| Synonyms |
Teratocarcinoma-derived growth factor 1;Cripto-1 growth factor;CRGF;Epidermal growth factor-like cri |
| Species |
Human |
| Predicted Molecular Mass |
42.8 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
31-169aa |
| Tag |
C-Fc |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Developmental Biology |
| Target Function |
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. |
| Subcellular Location |
Cell membrane; Lipid-anchor, GPI-anchor. Secreted. |
| Protein Family |
EGF-CFC (Cripto-1/FRL1/Cryptic) family |
| Tissue Specificity |
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. |
