| Product Name |
Recombinant Human Thymus Expressed Chemokine (TECK/CCL25) Protein |
| Product Overview |
This recombinant human Thymus Expressed Chemokine (TECK/CCL25) protein includes amino acids 24-150aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O15444 |
| Recommended Name |
C-C motif chemokine 25 |
| Gene Name |
CCL25 |
| Synonyms |
C-C motif chemokine 25 isoform 1, Ckb15, SCYA25, TECK |
| Species |
Human |
| Predicted Molecular Mass |
21 kDa |
| Expression System |
E.coli |
| Expression Range |
24-150aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology |
| Target Function |
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. |
| Subcellular Location |
Secreted. |
| Protein Family |
Intercrine beta (chemokine CC) family |
| Tissue Specificity |
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine. |
