| Product Name |
Recombinant Human CBR4 Protein |
| Product Overview |
This recombinant human CBR4 protein includes amino acids 1-237aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q8N4T8 |
| Recommended Name |
3-oxoacyl-[acyl-carrier-protein] reductase |
| Gene Name |
CBR4 |
| Synonyms |
Carbonyl reductase family member 4 |
| Species |
Human |
| Predicted Molecular Mass |
27.5 kDa |
| Expression System |
E.coli |
| Expression Range |
1-237aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Component of the heterotetramer complex KAR (3-ketoacyl-[acyl carrier protein] reductase or 3-ketoacyl-[ACP] reductase) that forms part of the mitochondrial fatty acid synthase (mtFAS). Beta-subunit of the KAR heterotetramer complex, responsible for the 3-ketoacyl-ACP reductase activity of the mtFAS, reduces 3-oxoacyl-[ACP] to (3R)-hydroxyacyl-[ACP] in a NADPH-dependent manner with no chain length preference, thereby participating in mitochondrial fatty acid biosynthesis. The homotetramer has NADPH-dependent quinone reductase activity (in vitro), hence could play a role in protection against cytotoxicity of exogenous quinones. As a heterotetramer, it can also reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro). |
| Subcellular Location |
Mitochondrion matrix. |
| Protein Family |
Short-chain dehydrogenases/reductases (SDR) family |
| Tissue Specificity |
Detected in liver and kidney (at protein level). Displays the highest expression in neuronal and muscle tissues. |
![SDS-PAGE analysis of Human CBR4 (3-Oxoacyl-[Acyl-Carrier-Protein] Reductase) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1grml13c71tpu1shrj.jpg?v=1755757497&width=1000)
![SDS-PAGE analysis of Human CBR4 (3-Oxoacyl-[Acyl-Carrier-Protein] Reductase) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1grml13c71tpu1shrj.jpg?v=1755757497&width=88)
![SDS-PAGE analysis of Human CBR4 (3-Oxoacyl-[Acyl-Carrier-Protein] Reductase) - Recombinant Protein, showing >85% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1grml13c71tpu1shrj.jpg?v=1755757497&width=640)
