| Product Name |
Recombinant Human Carbonyl Reductase 1 (CBR1) Protein |
| Product Overview |
This recombinant human Carbonyl Reductase 1 (CBR1) protein includes amino acids 2-277aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P16152 |
| Recommended Name |
Carbonyl reductase [NADPH] 1 |
| Gene Name |
CBR1 |
| Synonyms |
CBR, hCBR1, SDR21C1 |
| Species |
Human |
| Predicted Molecular Mass |
35 kDa |
| Expression System |
E.coli |
| Expression Range |
2-277aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
Short-chain dehydrogenases/reductases (SDR) family |
| Tissue Specificity |
Expressed in kidney (at protein level). |
![SDS-PAGE analysis of Human CBR1 (Carbonyl Reductase [Nadph] 1) - Recombinant Protein, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1hqc9jln21lob1uh5j.jpg?v=1755757497&width=1000)
![SDS-PAGE analysis of Human CBR1 (Carbonyl Reductase [Nadph] 1) - Recombinant Protein, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1hqc9jln21lob1uh5j.jpg?v=1755757497&width=88)
![SDS-PAGE analysis of Human CBR1 (Carbonyl Reductase [Nadph] 1) - Recombinant Protein, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.astorscientific.us/cdn/shop/files/1hqc9jln21lob1uh5j.jpg?v=1755757497&width=640)
