Product Name |
Recombinant Human Cadherin-1 (CDH1) Protein |
Product Overview |
This recombinant human Cadherin-1 (CDH1) protein includes amino acids 24-709aa of the target gene is expressed in Insect cell.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
Target Uniprot Id |
P12830 |
Recommended Name |
Cadherin-1 |
Gene Name |
CDH1 |
Synonyms |
CDH1, Arc-1, CD324, CDHE, ECAD, LCAM, UVO |
Species |
Human |
Predicted Molecular Mass |
76.6 kDa |
Expression System |
Insect Cell |
Expression Range |
24-709aa |
Tag |
N-6His |
Purity |
>90% |
Formulation |
Lyophilized |
Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
Research Area |
Signal Transduction |
Target Function |
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. |
Subcellular Location |
Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. |
Associated Diseases |
Hereditary diffuse gastric cancer (HDGC); Endometrial cancer (ENDMC); Ovarian cancer (OC); Breast cancer, lobular (LBC); Blepharocheilodontic syndrome 1 (BCDS1) |
Tissue Specificity |
Non-neural epithelial tissues. |