| Product Name |
Recombinant Human ASMT Protein |
| Product Overview |
This recombinant human ASMT protein includes amino acids 1-298aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P46597 |
| Recommended Name |
Acetylserotonin O-methyltransferase |
| Gene Name |
ASMT |
| Synonyms |
Acetylserotonin O-methyltransferase, HIOMT, HIOMTY |
| Species |
Human |
| Predicted Molecular Mass |
35.3 kDa |
| Expression System |
E.coli |
| Expression Range |
1-298aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity. |
| Protein Family |
Class I-like SAM-binding methyltransferase superfamily, Cation-independent O-methyltransferase family |
| Tissue Specificity |
Expressed in the pineal gland (at protein level). In the retina, very low expression is found at the mRNA level, and not at the protein level. |
