| Product Name |
Recombinant Human AICDA Protein |
| Product Overview |
This recombinant human AICDA protein includes amino acids 1-198aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9GZX7 |
| Recommended Name |
Single-stranded DNA cytosine deaminase |
| Gene Name |
AICDA |
| Synonyms |
Activation-induced cytidine deaminase, AID, ARP2, CDA2, HIGM2 |
| Species |
Human |
| Predicted Molecular Mass |
26.1 kDa |
| Expression System |
E.coli |
| Expression Range |
1-198aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. |
| Subcellular Location |
Nucleus. Cytoplasm. |
| Protein Family |
Cytidine and deoxycytidylate deaminase family |
| Associated Diseases |
Immunodeficiency with hyper-IgM 2 (HIGM2) |
| Tissue Specificity |
Strongly expressed in lymph nodes and tonsils. |
