Dispase II (Neutral Protease, Grade II) (TBS2117)

Description

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It can be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+, and other heavy metals inhibit enzyme activity.

Tribioscience’s Dispase II is a lyophilized powder, and stable at 2-8°C. It makes it easy to storage and application.

Main Features

• Rapid, effective, yet gentle agent that liberates cells with minimal cell damage.
• Maintains cell membrane integrity.
• Non-mammalian source – free of mycoplasma and animal virus contamination.
• Extremely stable to influences of temperature, pH, and interference by serum components.
• Easily inactivated by chelating agents or by dilution.
• Delivers higher activity and convenience.

Storage Conditions

Dispase II is stored at +2 to +8°C for a shelf-life of 1 year. The expiration date is printed on the label.