Thiol Fluorometric Assay (TBS2026)

Description

Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The detection and measurement of free thiols is one essential task for investigating biological processes. Thiols are extremely efficient antioxidants that can protect cellular lipids, proteins, and nucleic acids against peroxidative damage due to their strong reductive capacity and their ability to react with free radicals.

Tribioscience’s Thiol Quantification Fluorometric Assay Kit provides a flexible, accurate, sensitive, and time-saving approach for detecting FREE thiol groups in a wide variety of samples.

In this assay, the non-fluorescent probe binds to the thiol in the sample to produce a fluorescent product which can be measured at 400 nm excitation, 460 nm emission.

Features

• Flexible: Suitable for 96-well and 384-well plate.
• Accuracy: Fluorescent measurement is proportional to the free thiol content.
• Sensitive: detection range from 0.3 µM to 20 µM.
• Time-saving: Just incubate for 10 minutes and read out style.

Applications

• Measure free thiol group in a variety of samples like cells and tissues.

Typical GSH Standard Curve