β-Glucuronidase Assay (TBS2110)

Description

β-Glucuronidases are hydrolytic enzymes responsible for the breakdown of carbohydrates. Specifically, β-Glucuronidases cleave the terminal β-D-glucuronic acid residue from the nonreducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Glucuronidase activity results in metabolic disease and health problems. It is important to detect β-Glucuronidase activity in the tested samples for disease examination.

The Beta-Glucuronidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring glucuronidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of glucuronidase activity in a variety of samples.

Applications

This kit is used for the determination of β-Glucuronidases activity in biological samples.

Key features

• Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30 minutes reaction at 37˚C.
• High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Preparation

Standard Curve

Related Products

1. Tryptase activity colorimetric assay (TBS2101)
2. Hex activity colorimetric assay (TBS2105)
3. Caspase-3 Fluorometric Assay kit (TBS3230)
4. Cytochrome C Oxidase Activity Assay (TBS2115)
5. Fast Glucose Determination Colorimetric/Fluorometric Assay (TBS2087)