{"product_id":"1x-rbc-lysis-buffer","title":"1x RBC Lysis Buffer","description":"\u003cdiv class=\"product attribute overview\"\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003eBuffer for the gentle lysis of red blood cells (RBC, erythrocytes)\u003c\/div\u003e\n\u003cdiv class=\"value\" itemprop=\"description\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv id=\"product.info.description\"\u003e\n\u003ch2\u003eDetails\u003c\/h2\u003e\n\u003cdiv class=\"product attribute description\"\u003e\n\u003cdiv class=\"value\"\u003e\n\u003cp\u003eRed Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. Nucleated RBCs are not effectively lysed with ammonium chloride. This 1x RBC Lysis Buffer is ready to use.\u003c\/p\u003e\n\u003ch3\u003eComponents\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eNH\u003csub\u003e4\u003c\/sub\u003e Cl (155 mMol\/L)\u003c\/li\u003e\n\u003cli\u003eKHCO\u003csub\u003e3\u003c\/sub\u003e (10 mMol\/L)\u003c\/li\u003e\n\u003cli\u003eEDTA (0.1 mMol\/L)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eEnsure that the RBC Lysis Buffer is cold (4-8°C) .\u003c\/li\u003e\n\u003cli\u003eDilute one volume of cell suspension with 10 volumes of 1× Red Blood Cell Lysis Solution.\u003c\/li\u003e\n\u003cli\u003e\n\u003ci\u003eWhen working wih blood samples:\u003c\/i\u003e\u003cbr\u003eVortex for 5 seconds and incubate for 10-15 minutes (no more than 15 minutes) at 4-8°C.\u003cbr\u003e\u003cbr\u003e\u003ci\u003eWhen working with tissue samples:\u003c\/i\u003e\u003cbr\u003eVortex for 5 seconds and incubate for 2 minutes at 4-8°C.\u003cbr\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Observe turbidity to evaluate red blood cell lysis. Once the sample becomes clear red, lysis is complete.\u003c\/li\u003e\n\u003cli\u003eVortex for 5 seconds and centrifuge at 300×g for 10 minutes at room temperature.\u003c\/li\u003e\n\u003cli\u003eAspirate supernatant completely.\u003c\/li\u003e\n\u003cli\u003eResuspend the cell pellet in an appropriate buffer and proceed to further applications.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"additional\"\u003e\n\u003ch2\u003eMore Information\u003c\/h2\u003e\n\u003cdiv class=\"additional-attributes-wrapper table-wrapper\"\u003e\n\u003ctable class=\"data table table-striped additional-attributes\" id=\"product-attribute-specs-table\"\u003e\n\u003ccaption class=\"table-caption\"\u003eMore Information\u003c\/caption\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eDelivery Time (days)\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Delivery Time (days)\"\u003e1-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eArea of interest\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Area of interest\"\u003eCell Biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eSterility\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Sterility\"\u003eSterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003epH Range\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"pH Range\"\u003epH 7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eShipping Condition\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Shipping Condition\"\u003eRoom Temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eStorage Condition\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Storage Condition\"\u003e4-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth class=\"col label\" scope=\"row\"\u003eRegulatory Statement\u003c\/th\u003e\n\u003ctd class=\"col data\" data-th=\"Regulatory Statement\"\u003eFor research use only. Not for use in diagnostic procedures.,\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AstorScientific","offers":[{"title":"100 ml","offer_id":67875791896653,"sku":"PS60-00050-11","price":39.6,"currency_code":"USD","in_stock":true},{"title":"250 ml","offer_id":67875791929421,"sku":"PS 60-00050-12","price":79.2,"currency_code":"USD","in_stock":true},{"title":"500 ml","offer_id":67875791962189,"sku":"PS60-00050-13","price":138.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0568\/5883\/7069\/files\/1xrbc_lysis_buffer_500_kopie.png?v=1793553327","url":"https:\/\/www.astorscientific.us\/products\/1x-rbc-lysis-buffer","provider":"AstorScientific","version":"1.0","type":"link"}